Where do you see the direction of quantitative microscopy moving in the future?

Prime, contains a much more sophisticated package of image analysis tools that really enables quantitative applications in microscopy. These applications include nuclear translocation, fluorescent in-situ hybridization, co-localization studies, and cell cycle analysis, among others.
Where do you see the direction of quantitative microscopy moving in the future? Both in terms of improvement in the imaging software and in microscopy automation?
I think BioTek is now established as a leader in automated quantitative microscopy. We will continue to further this reputation as we build out our capabilities in simplifying microscopy through automation and enhancing the phenotypic assay capabilities that Image Prime enables.

One area where I think automated quantitative microscopy can be extremely beneficial is applied to 3D cell culture methods. Scientists are moving away from imaging a layer of 2D plated cells, whether on a microscope slide or microplate well.

Extensive research is being performed with structures like hydrogels, spheroids, tissues and organ on chip devices. I think automated quantitative microscopy can better enable these physiologically relevant types of assays, where the experiment sample more closely resembles what's going on in human beings
Peter Banks is the Scientific Director at BioTek Instruments, Inc. His responsibilities include the management of the company’s applications team and providing scientific guidance to the senior management team on new technology and emerging trends impacting the microplate market and its peripheral markets of liquid handling and detection.

Before joining BioTek in 2008, Banks was employed in a number of roles within PerkinElmer for a decade. These roles included management of PerkinElmer’s BioPharma R&D program and Director of Molecular Pharmacology for PerkinElmer BioSignal in Montreal, Canada, focusing on the development of numerous detection technologies and reagent systems for High Throughput Screening.

Prior to experiences at PerkinElmer and BioTek, Banks was an Assistant Professor in Analytical Chemistry at Concordia University, Montreal, Canada from 1994 to 1998. Banks was awarded an NSERC post-doctoral fellowship in 1993 following his dissertation for PhD at the University of British Columbia in 1992.
Do you provide image processing software that can be used with your microscopes?
I want to start by making a difference between imaging processing and imaging analysis. To my mind, imaging processing involves background reduction, smoothing, stitching a montage of images together or taking a Z-stack and projection through thick biology.

Conversely, image analysis uses various algorithms to quantify biology after acquiring and processing that image. We have two different levels of image analysis software to meet specific analysis requirements.

Gen5 Image+ allows you to perform simpler applications like counting cells, measuring transfection efficiency of GFP, for example or determining % confluence.

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